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There was no HSP90B1 signal visible in MT extracts while alpha-tubulin was similarly detected in MT as in WT samples.
For proteins in common between total and mt extracts, the protein turnover rates were in high agreement (Fig. S5, Spearman's ρ > 0.65 with P < 0.001 for all slope comparisons), demonstrating the high reproducibility of the turnover rate measurements.
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WB planned and performed the analyses, and drafted the manuscript, BH bled cattle, extracted DNA, RB bled cattle, extracted DNA, MT extracted DNA, LT, obtained cattle, led the bleeding of cattle.
This outcome of our microarray analysis is astounding if we consider that the 9c and 30c dilutions were obtained from MT extract by dilution factors of 10 and 10 respectively.
Two authors (TAV and MT) independently extracted data using a predetermined template.
Based on the MT parameters (extracted using a data reduction technique), three typical asymmetrical postural patterns were described: (1) the postural pattern of children with mild scoliosis (SCOL), (2) the progravitational postural pattern (PGPP), and (3) the antigravitational pattern.
For example, in Xenopus egg extracts, MT dynamics are determined by a balance between the MT growth-promoting XMAP215 and MT-destabilizing XKCMI (Tournebize et al., 2000; Kinoshita et al., 2001).
SDS-PAGE analysis of MT-I and MT-II crude extracts from the transformed E. coli (M15) showed high amounts of a polypeptide with the expected molecular mass (ca. 6.5 and 8 kDa) (Figure 3A and 3B).
SDS-PAGE analysis of MT-I and MT-II crude extracts from the transformed E. coli (M15) showed high amounts of a polypeptide with the expected molecular mass (ca. 6.5 and 8 kDa, respectively) (Figure 1).
Coding sequences annotated as orthologues of the human MT genes were extracted from the Ensembl database (www.ensembl.org, release 56: Sep 2009) [19].
MT and HS extracted genomic DNA and performed sequencing.
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