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The control plasmid allowing the expression of MT without Hfq was created by inserting the MT DNA sequence directly after the BAD promoter without Hfq.
The MT DNA sequence was produced by fusion-PCR.
This procedure allowed the mt DNA sequence to be acquired nonetheless at an acceptable fold-coverage.
Nuclear protein-coding sequences and the reference N. lolii mt DNA sequence have been deposited in GenBank.
This study would provide baseline information for precisely describing the macrohaplogroup M, as more complete mt DNA sequence information is generated.
More recently, mt DNA sequence data has been used to explore the peopling of Asia and to comprehend various population demographic parameters [ 12- 20].
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The mt DNA sequences of S. japonicum samples from south-western (SW) China were 2 bp [position 11727 11728 within tRNA-Cys, microsatellite (AG) indel] longer than those of the parasites from the lower Yangtze/Zhejiang areas.
We obtained complete mt DNA sequences from the NCBI genome database.
Table S3 gives the values of Thr/Cys compositions and Log (MLS) in primates and the accession number of mt DNA sequences used in this analysis.
Comparative genomics of Mt DNA sequences from five species of Tetrahymena allowed us to identify a putative transcription control region common to all these genomes.
The mt DNA sequences were extracted from various whole genome sequence libraries.
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