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Total RNA was extracted from the pericarps of WT and MT as previously described [ 30].
This probably reflects much NMD of the transcript in human Mb and Mt, as previously observed for C2C12 cells.
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Isolation of Mt DNA was as previously described [31].
Early log-phase ribosomes from P. aeruginosa strain PA01 were prepared in the laboratory of Walter Hill at the University of Montana (Missoula, MT-) as previously described [ 15].
The mt genome phylogram reinforced this distinction, suggesting that the UNS mt genome may have been contributed by the ancestor of the FGC1/LAE lineages, while the FaTG-2 mt genome, as previously described, is most closely related to E. festucae.
Accordingly, we constructed 2D MT maps, where each MT's chain membership was displayed, and we compared these to the heat maps of local packing as previously described.
with a loadcell of 5 kN, and the data were recorded using computer software (Nexygen-MT; Lloyd Instruments) as previously mentioned in the evaluation of SSBS.
Tetrode recordings, by using a Digital Lynx system (Neuralynx, Bozeman MT), were performed as previously described (Ji and Wilson, 2007, 2008).
The PyV MT cell lines were generated from PyV MT tumors and cultured as previously described in complete DMEM [ 9].
The telomeres on each terminus of the T.pigmentosa Mt genome were different as previously found by Morin and Cech [24], [25].
Whole liver tissue was homogenized, or mt fractions were isolated as previously described (Zhang et al., 2008).
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