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Restriction digest for the DNA fragment was carried out using Msp 1 restriction enzyme.
*Adjusted for age and waist/hip ratio (WHR) The DNA extraction and RFLP Msp 1 assays were successful in 114 premenopausal breast cancer cases and 121 control subjects.
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These studies suggested that Msp-2 carriers were more susceptible to lead effects than were Msp 1-1 homozygotes.
Wu et al. (2003) postulated that lead toxicity became apparent at a lower exposure level for Msp-2 carriers than for Msp 1-1 persons.
Bergdahl et al. (1997) found that serum creatinine is higher for Msp-2 carriers than for Msp 1-1 homozygotes in a sample of 89 lead workers.
These may be suitable for the development of MSP-1-based vaccines against malaria.
Multistage immunity against malaria can thus be achieved by using viral vectors recombinant for MSP-1.
The genotyping of P. falciparum showed that K1+MAD20 mixed genotype was dominant in msp-1 and FC27 in msp-2.
Merozoite surface protein 1 (MSP-1) is a precursor to major antigens on the surface of Plasmodium spp. merozoites, which are involved in erythrocyte binding and invasion.
In vivo tests revealed that reduced amide pseudopeptide-immunised Aotus monkeys induced neutralising antibodies specifically recognising the MSP-1 N-terminus region.
The C-terminal portion of the Plasmodium falciparum blood stage MSP-1 antigen plays a key role in invasion of human erythrocytes.
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