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Due to the diffusive tendency of the GFP-MYA2-head6IQ, exposure times between 300 and 700 msec were used for widefield fluorescence microscopy proving to be a fast and appropriate method for studying its distribution.
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Same exposure time (20 mSec) was used for image acquisitions of WT and Cx30 null mice.
To ensure fluorescent intensities are comparable across different samples, a constant exposure time (300 mSec) was used.
Abnormal or prolonged QRS duration (>120 msec) is used in the diagnosis of bundle branch block or ventricular rhythm.
A repetition time of 1.5 sec was used yielding an acquisition time of 14.4 min. An echo time of 40 msec was used to minimize spectral overlap of NAA, Cr, and Ch with amino acids and macromolecule resonances.
13C NMR, JMOD-1H and COSY, TOCSY, HSQC, HSQC-EDITED, HMBC (3 J: 7 and 10 Hz), NOESY (mixing time at 100, 200, 300 msec) experiments were used for structural determination.
To match previous animal and human studies, gaps of 50-msec duration were used.
For the analysis of sensory-evoked oscillations, 500 msec periods following SEP were used.
For baseline activity assessment periods 200 msec prior to stimulus were used.
3 mm T1-weighted sequences (TR 600 msec, TE 14 msec, Matrix 256×192, FOV 250 mm) were used for volumetric measurements of the olfactory brain.
60-min MRM methods were used with fixed 10 msec dwell time for each transition and "unit" resolution for both Q1 and Q3 quadrupoles.
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