EOG correction (Gratton et al. 1983), artifact rejection (± 100 μV), and baseline correction (100 msec) were applied.
A normalised collision energy of 35%, an activation of q = 0.25, and activation time of 30 msec were applied in MS/MS acquisition.
Condensation acoustic clicks, 0.01 msec, were applied with a distance of 12 inches from the subject at a sound level of less than or equal to 80 dB SPL in one ear with the contralateral ear plugged.
2-PE was identified with gas chromatography mass spectrometry (GC-MS; Agilent 6890 N GC and 5975 inert MSD) using a pulsed pressure injection of 1 μl onto an Innowax-101025 column (30 m × 0.25 mm, 0.25 μm film thickness), ion source (EI) ionization, and a dwell time of 40 msec were applied for each characteristic ion.
At fixed intervals of 1 min a transepithelial bipolar current pulse (I) of 1 µA amplitude and 200 msec duration was applied to the tissue and the RT was automatically calculated from the change in open-circuit voltage (ΔVT) according to Ohm's law (RT = ΔVT/I).
Cells were held at a holding potential of −80 mV before a step protocol of 25 msec or 50 msec to different voltages was applied to determine the current-voltage relationship.
A sequence of seven radiofrequency pulses of 500 msec, called neuropsychophysical optimization, was applied by touching the metallic tip of the REAC probe onto seven reflex auricular points.
Next, the individual BMP-2 and BMP-7 plasmids or both together were injected into rat calf muscles, and transcutaneous electroporation was applied 8 times at 100 V, 50 msec.
Immediately after DNA administration, an electrode was placed over the injection site and voltage was applied (2 pulses, 1125 V/cm, 50 µsec+8 pulses, 275 V/cm, 10 msec).
The spacing of the TriGridTM electrode array was 6 mm in a diamond-shaped configuration, and the electrical field was applied at an amplitude of 250 V/cm of electrode spacing for a 40 msec total duration applied as three pulses over a 400 msec interval, resulting in brief deltoid muscle contractions.
