Stimulation pulses of 0.2-msec duration and up to 10 V in amplitude were delivered by a Digitimer DS2 stimulator (Digitimer, Welwyn Garden City, UK) via a Powerlab (ADInstruments Oxford, UK), driven by Scope software running on Apple Mac Powerbook.
Number of GFP molecules per pixel was computed (∼7700) and compared to the mean pixel fluorescence (∼3000 fluorescence A.U. per 1000 msec exposure), and summed up to ∼0.4 fluorescence counts per molecule.
T1-weighted structural volume using 3-D MP-RAGE sequence (TR = 11 msec; TE = 4.94 msec; flip angle: 158; FOV: 256×256 mm2; up to 176 axial slices, ensuring whole brain coverage, parallel to AC PC line; slice thickness: 1 mm; interslice gap: 0 mm resulting in 1×1×1 mm voxels) was acquired following the runs for each of tasks.
For this analysis, we used a step size of 100 msec and analyzed the data up to 100 Hz.
The performance difference in relation to somatotopic distance was present for retention intervals of up to 800 msec, indicating that the direct involvement of SI in the retention of frequency information is temporally limited.
Interictal discharges were measured as fast and high amplitude spike events lasting up to 200 msec.
Conduction velocities of recorded units were estimated from conduction latency values recorded between electrical monopolar stimulation (up to 30V for 0.5 msec at 0.3 Hz) of their receptive field and the onset of evoked action potentials, and conduction distances were estimated along the nerve between the stimulation and recording sites (31).
To estimate an effective diffusion, MSD values for lag times of up to 10 frames (1 frame = 30 msec) were fitted with the equation for a particle diffusing in two dimensions [ [ 〈 Δ R → 2 (t ) 〉 = 4 D t ] ] (Berg, 1993).
For the analysis of the high-gamma frequency range (up to 300 Hz), we used a time window of 100 msec with a step size of 50 msec.
Indeed, in the ECHO study, QTcF increased over time up to week 48 in both the rilpivirine and efavirenz groups, with no relevant differences between the means (10.9 msec versus 12.0 msec), 3 and only three grade 1 adverse events were reported that might have been related to conduction abnormalities or to rate or rhythm disturbances.
