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In addition, a mixing time of 80 ms and a trim pulse length of 2500 ms were set for the TOCSY experiment.
Cut-off points of 55.45 and 36.64 ms were set for LF and HF, respectively.
The recycle delay of 2 s, t1 of 4 μs, and the mixing time (tm) of 100 ms were set.
For FT MS/MS spectra an AGC target value of 5E5 and an injection time of 200 ms were set.
Cut-off points of 356.13, 55.45 and 36.64 ms were set for total power, low frequency and high frequency (HF), respectively.
Criteria for positive identification of proteins with MS were set according to the scoring algorithm delineated in Mascot (www.matrixscience.com).com
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The noise level for Δt = 250 ms was set to ±8%.
A time window of 1800 ms was set with activity in the 300 ms preceding the picture presentation serving as the baseline for measurement.
Total intensity of each data bin, 30 ms, is set to be 500 a.u., matching our typical data intensity in TIRF experiments.
An accumulation time of 100 ms was set for each fragment ion resulting in a total duty cycle of 3.3 s.
Small mammal plots (10 m × 10 m) were set on site with mouse- and squirrel-size live traps for 3 days during 4 weeks in early fall.
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