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2D TOCSY experiments [41], [42] were performed on each peptide with a mixing time, τm, of 20 and 70 ms. Four 2D-NOESY experiments with mixing times ranging from 100 to 500 ms were performed.
In cardiomyocytes the time series of the fluorescencent probes, consisting of 2000 to 4000 images with temporal resolution of 110 ms to 120 ms, were performed with the couples TMRM-CMH2DCF or MitoSox-NADH.
Ventricular ERP was determined at a drive cycle length of 100 ms. Programmed ventricular stimulation, consisting of burst pacing at cycle lengths of 100 ms to 50 ms in decrements of 10 ms, and of programmed stimulation with double and triple extrastimuli at a drive cycle length of 100 ms with a coupling interval ≥30 ms, were performed.
Two pulses at 1150 V for 30 ms were performed.
T1-weighted sequences (3 mm slices, FOV 250, TR 700 ms) were performed without and with 0.1 mmol/kg bodyweight Gd-DTPA (3 mm slices, TR 3000 ms, TE100 ms).
Analyses of peptides by MALDI-TOF2 MS were performed on an Axima Performance mass spectrometer (Shimadzu Biotech, Japan) equipped with a 337 nitrogen laser and a collision-induced dissociation (CID) chamber with helium gas.
Similar(39)
As part of LES work, a dwell time variation of 0.65 ms (0.3/0.65/1.2 ms) was performed to reveal the sensitivity of soot production to variations in dwell time.
A block of 20 trials (with an intertrial interval varying randomly between 1500 and 2000 ms) was performed for each index finger.
High resolution time-bin analysis (5 ms) was performed to give a more fine-grained temporal characterization, at each electrode separately.
Underwater blast loads using 1.28 kg TNT equivalent charge at a stand-off distance of 1 m were performed on four different composite sandwich panels.
Amplifications for NA or β2-m were performed separately.
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