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Movements with a duration of less than 200 ms were considered irregular, as were movements for which the total trajectory was longer than 5 times the target amplitude.
Trains of action potentials preceded by a silence period of at least 50 ms and whose interspike intervals were less than 4 ms were considered as bursts [23].
Only spikes separated in the range from 0 to 20 ms were considered for the analysis because almost all interspike intervals during the transient phase of dLGN cells response fell into this range (see Material and Methods).
Half AP widths broader than 0.8 ms were considered as 0.8 ms. For the calculation of dopamine neuron's burst firing probability, baseline activity when mice were free behaving was used according to previous established criteria (burst onset, ISI of ≤80 ms; burst offset, ISI of ≥160 ms) [50].
Trials with reaction times smaller than 200 ms were considered as anticipations and excluded from the analysis, on the basis of Go/NoGo studies which show that in their context, reaction times cannot be produced faster than 250 ms [19], [20].
For the analysis of the response times, response times below 200 ms were considered outliers.
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Maximum delay of 1 ms is considered.
Drugs that prolong the QT interval for more that 20 ms are considered proarrhythmic and are generally not marketed [2].
Thus a temporal discrepancy of more than 500 ms is considered to be sufficient to reduce RHI effects.
The time interval from −90 ms to 20 ms was considered, which was 110 ms in duration and contained 22 sample points.
In general, an interval of above 480 ms is considered prolonged.
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