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To reduce the impact of localisation error on these results a temporal Gaussian smoothing filter of FWHM 4 frames (200 ms) was applied to the position traces before the colocalisation analyses.
Electrostimulation of 36∼80 V, 25∼80 Hz with the pulse time of 0.7 ms was applied across the chelicerae.
A second stimulation pulse with high light intensity (8.4 mW, 1000 ms) was applied after a 2.5 min interval (Fig. 4E).
In a separate series of experiments, twin-pulse electrical stimulation (ISI = 40 70 ms) was applied to the cutaneous terminals of 46 mechanically-sensitive nociceptive afferent fibers (MSAs) and of 36 mechanically-insensitive afferent/efferent fibers.
Stimulus duration of 0.3 ms was applied as cut-off value.
An activation q = 0.25 and activation time of 30 ms was applied.
Similar(32)
Gas chromatography negative chemical ionization-mass spectrometry (GC NCI-MS) was applied for sample detection.
Coupled size exclusion chromatography (SEC)–electrospray ionization mass spectrometry (ESI-MS) was applied to carefully map the product spectrum of a series of acrylate free radical polymerizations mediated via the reversible addition fragmentation chain transfer (RAFT) process.
Focused ultrasonic solid liquid extraction (FUSLE) and reverse-phase ultra performance liquid chromatography (UPLC) coupled to a quadrupole-time of flight mass spectrometer (Q-TOF-MS) was applied to the determination of bisphenol-type endocrine disrupting compounds (EDCs) in food-contact recycled-paper materials.
Only for the peptidomics markers, CE-MS was applied for all phases.
ICP-MS was applied for further analysis of the cations of the samples.
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