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The pause time was 5 ms, scan speed was 0.56 sec/scan, and corona discharge, -4.
T1 (TR/TE: 1000/10.6 ms; scan time 12 min) and T2-turbo-rare sequences (TR/TE: 6351.6/75.6 ms; scan time 12 min) were performed with a resolution of 176 × 176 μm and a slice thickness of 500 μm.
MS scan time: 1 + 0.1 s interscan delay.
The injection volumes, MS scan range were 1 µL and 30 600 m/z, respectively.
The MS scan was configured to Multiple Ion Detection (MID) to simultaneously measure compounds of different masses.
Data was collected in the "Triple-Play" (MS scan, Zoom scan, and MS/MS scan) mode.
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The LC-MS scan demonstrated presence of different compounds showing 3 20 min retention time.
The LC-MS scan demonstrated presence of different compounds showing 3 20 min retention time (RT).
The lower panel shows fragmentation of d17iso-SPA by MS-MS scan.
After a delay of 800 ms, an MS or MS/MS scan is performed at 1 mTorr.
The injection times were 50 ms in a full scan and 200 ms in a MS/MS scan.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com