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An integration of 33 ms per line gives an acquisition time of 22 s per image cube.
The first spectrometer utilized an EMCCD detector (Cascade II, Photometrics) operating from 380 to 1000 nm with 5 nm sampling, at an illuminance of 1100 lx and integration time of 50 ms per line.
The second spectrometer utilized an InGaAs array (SUI 640SDV, Sensors Unlimited) operating from 967 to 1680 nm with 3.4 nm sampling, 650 lx, and integration time of 100 ms per line.
The second spectrometer utilized is an InGaAs array (SUI 640SDV, Sensors Unlimited) operating from 967 to 1680 nm with 3.4 nm sampling, ~1000 lux, and an integration time of 30 ms per line.
The scan speed was 1.5 ms per line pair.
Linescans were obtained at an interval of 1.33 or 0.833 ms per line; x-y images (128 pixels×30 or 40 lines) were recorded at an average frame rate of 37.5-, 44-, or 57.3-ms intervals.
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The microwaves are guided from the gyrotrons via (ø50 mm) waveguide transmission lines, into the plasma via the First Confinement System (FCS), consisting of a 'Z'-shaped set of straight corrugated aluminum waveguides, totaling ∼13 m per line, connected by miter bends (MB).
Dwell time was 145 ms per channel.
The NFFT processing speed of 5.7 ms per 512 A-lines corresponds to using 11.1 us to process a single A-line.
Whole protein was extracted by M-PER Mammalian Protein Extraction Reagent from cell lines added with Phosphatase Inhibitor Cocktail Set II (Calbiochem, San Diego, CA) and Complete Protease Inhibitor Cocktails (Roche, Lewes, UK) according to manufactures' protocols.
Whole cell lysates in A-204 and SJSA-1 cell lines were obtained with M-Per Mammalian Protein Extraction Reagent (Pierce, Rockford, IL).
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