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The transcriptional response was used to identify key areas in metabolism around which mRNA expression changes are significantly associated.
To identify metabolites in yeast around which mRNA expression changes are significantly associated, we applied the Reporter Metabolite Algorithm [ 18] (see Methods).
We observed a high degree of concordance between the mRNA expression changes with I-BRD9 treatment and Brd9 knockdown.
We treated ESCs with I-BRD9 and assessed mRNA expression changes at 24 and 48 h post treatment using high-throughput sequencing (RNA-Seq).
Using this macroarray, shear stress-induced mRNA expression changes were analyzed in human coronary artery endothelial cells.
Treatment of RLE/Abca3 cells with bleomycin (BLM) and methotrexate (MTX) induced similar morphological and mRNA expression changes.
The profile of hepatic transporter mRNA expression changes in PMA-treated HepaRG cells was correlated to that found in PMA-exposed primary human hepatocytes and was similarly observed in response to the PKC-activating marketed drug ingenol mebutate.
This study demonstrates, for the first time, that steroid sulfatase is localized to human epithelial cells and that steroid sulfatase staining and mRNA expression changes with the menstrual cycle.
They are also associated with mRNA expression changes in ORMDL3.
(A and B) Genetic alterations and mRNA expression changes of the USP33 in the TCGA dataset of lung cancer samples.
The tendencies of mRNA expression changes were in accordance with the RNA-Seq analysis results, which well-validated of the sequencing data.
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