Sentence examples for mp buffer from inspiring English sources

To test PKA phosphorylation of purified AQP0, 2.5 µg AQP0 was incubated for 1 h in 50 µl MP buffer containing 10 µCi/µM P-γATP and supplemented with 250 U PKA C subunit, and 0.1 µg PKI as appropriate.

Mycobacterium avium subsp. paratuberculosis at an OD600 of ~0.6 were transduced with ~3 × 10 phages in MP buffer (50 mM Tris-HCl [pH 7.6], 150 mM NaCl, 2 mM CaCl2) for 4 hours at 37°C, transferred to 7H9 medium for 24 hours with rotation at 37°C, and subsequently plated on selective 7H10 medium.

Finally, the high titer transducing mycobacteriophages were prepared (10 plaque forming units PFU/mL) and mixed with an equal volume of a MAA culture with an optical density (OD) at 600 nm of 0.6, which had been previously centrifuged and resuspended in MP buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 10 mM MgCl2, 2 mM CaCl2).

The M. tuberculosis library was prepared by washing an M. tuberculosis GC1237 culture, of approximate OD600 1.0 with an equal volume of MP buffer and resuspending in 0.1 volumes of phAE87 at an multiplicity of infection (MOI) of between 10 100, incubating at 37°C for 4 h and plating on 7H11 media containing kanamycin at 30 μg ml−1.

After extensive washes in PBS, arrays were incubated with anti-His conjugated to horseradish peroxidase (A7058; Sigma-Aldrich) diluted 1 500 in MP-PBS buffer.

After extensive washes in PBS, arrays were incubated with anti-rat conjugated to horseradish peroxidase (A7058; Sigma-Aldrich) diluted 1 1000 in MP-PBS buffer.

Each tissue sample was weighed and ground in 10% Trichloroacetic acid buffer (MP Biomedical).

The restriction products were fractionated on 2% agarose gels (Agarose, MP, Sigma) with TBE buffer (0.089 M Tris, 0.089 M boric acid and 0.5 M EDTA, pH 8.0), stained with ethidium bromide, and observed under UV light.

Pre-amplification was performed in a mixture containing 4 μl of the above reaction, 2.5 pmol of each E01 and HM0 primers (Table 5), 200 μM dNTPs (MP Biomedicals), 1× PCR buffer and 0.5 U Taq DNA Polymerase (MP Biomedicals) of a final volume of 20 μl.

Unbound detection reagent was removed by washing (four times) with assay buffer, and MPs were transferred to a new plate.

The subculturing process was initiated by removing the used medium followed by rinsing the cells twice with Phosphate buffer solution (PBS) (MP Biomedical, France).