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The two-photon images in Figures 1G and H were acquired in the same piece of tissue, simply by moving the microscope objective along the z direction.
We chose the sampling area for background silver grains by moving the microscope stage until the tissue was no longer visible.
A second image is captured (without moving the microscope's objective) to serve as the undeformed or reference configuration.
This solution allows moving the microscope head maintaining the laser beam aligned and centered inside the arm itself.
The bead was then pulled away, forming a membrane tether (>10 µm length), by moving the microscope stage under computer control at a constant velocity.
Both the cantilever and pillar were made to bend by moving the microscope platform, and with it the pillar, towards the cantilever at a predefined constant velocity.
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You can't move the microscope stage, but some technologies allow you to reposition the objective.
In addition, the software records the positions of fluorochrome-positive cells on the slide and can move the microscope stage to re-localise positive cells for direct assessment in the microscope.
To rule out that the bead had not simply detached, we moved the microscope stage and observed an increase in force indicating an intact microtubule-motor connection (see silver asterisk in Figure 5D).
Researchers have to count both live and dead yeasts in the small square of the hemocytometer, move the microscope stage to cover a neighboring square and count live and dead yeasts in the next square.
Move the microscope turret so the objective lens clicks into place, touching the oil.
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