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The five optimization functions are selected to describe the moving objectives.
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(b) Brightfield images captured on the VL21 microscope acquired at various focal positions by moving objective in the z axis.
Sphere samples adsorbed to a cover glass were sometimes translated on a piezo stage to alter the distance from sample to objective along the optical axis with nanometer precision when a moving objective was undesirable (nanopositioners from MCL, Madison, WI).
The inertia of the moving objective clearly restricts the z-scanning speed of these approaches.
The chamber is designed to fit into a standard, humidified incubator in an atmosphere of 5% CO2, and a moving objective allows the cell culture to be stationary while images are captured at different positions from well to well.
While new 2D scan techniques for in vivo population imaging achieve sampling rates from ten up to several hundred Hertz [ 5], typical maximum 3D scanning rates are lower, mostly due to the limit set by the inertia of the moving objective.
Well-run meetings allow you and your team to clarify issues, set direction, and move objectives forward.
The model can provide the conditions for crowd optimization of moving objects by the objective state of different time domain.
That's just what you can do with the Radially Moving Objective, or RMO, from MBF Bioscience in Williston, Vermont.
The analysis focused on four moves: objectives, methodology, results and conclusion.
The computer-controlled routine involves sequential steps of moving the objective lens relative to the sample to find the objective position that results in maximum variance in intensities within the field.
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