Exact(5)
Sphere samples adsorbed to a cover glass were sometimes translated on a piezo stage to alter the distance from sample to objective along the optical axis with nanometer precision when a moving objective was undesirable (nanopositioners from MCL, Madison, WI).
The inertia of the moving objective clearly restricts the z-scanning speed of these approaches.
The chamber is designed to fit into a standard, humidified incubator in an atmosphere of 5% CO2, and a moving objective allows the cell culture to be stationary while images are captured at different positions from well to well.
While new 2D scan techniques for in vivo population imaging achieve sampling rates from ten up to several hundred Hertz [ 5], typical maximum 3D scanning rates are lower, mostly due to the limit set by the inertia of the moving objective.
That's just what you can do with the Radially Moving Objective, or RMO, from MBF Bioscience in Williston, Vermont.
Similar(55)
The five optimization functions are selected to describe the moving objectives.
The model can provide the conditions for crowd optimization of moving objects by the objective state of different time domain.
The computer-controlled routine involves sequential steps of moving the objective lens relative to the sample to find the objective position that results in maximum variance in intensities within the field.
The measurement plane (i.e., object plane) was precisely positioned relative to the test section by moving the objective lens vertically in the y direction and by moving the table horizontally in the x and z directions.
The measurement plane (i.e., the object plane) was precisely positioned relative to the test section by vertically moving the objective lens in the y direction and by horizontally moving the table in the x and z directions.
Therefore, focusing of the traditional microscope requires moving the objective, the tube, and the eyepiece as a rigid unit.
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