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A moving chemoattractant source may attract the cell over a long distance.
However, such a strategy requires the moving chemoattractant source to be initially in the cell detection range and moves at low speed relative to the cell (File S1).
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A free beads assay was designed as a variation of the pipette assay and is suitable to check if cells can change their direction of migration to track a moving source of chemoattractant.
We consider the steady-state pattern of messenger molecules produced in the membrane of a cell perceiving and responding to an extracellular gradient of chemoattractant, which directs cell movement towards the chemoattractant source.
Experimental studies of neutrophil migration reveal that cells preferentially migrate toward a distant chemoattractant source in two competing chemoattractant gradients [12], [13], [14], [15], [16].
Dictyostelium cells extend one or more pseudopodia at a time, with the extended pseudopodia being close to the chemoattractant source.
Chemotactic cells such as neutrophils have the ability to sense, orient themselves and migrate toward a chemoattractant source even if the chemoattractant concentration difference is as small as a few per cent across the cell [11].
The chemoattractant was added before the start of imaging, and the chemoattractant source is due south of the movie.
As a result, the leading edge becomes stabilised in the direction of chemoattractant source.
The formation of filopodia precedes further polarization such as directed bleb formation and onset of migration towards the chemoattractant source.
The chemoattractants used in this study include human recombinant EGF (Invitrogen) at final concentration of 25 n M, as well as 10% FBS serving as a general chemoattractant source.
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