To analyze the contribution of different factors on biased cell movement, we tested three methods of representing persistence in the model.
Since F-actin organization is one of the key effectors of cell movement, we tested the effect of inhibition of Wnt-signals on the F-actin organization in TNBC cells.
To minimize smooth-pursuit related dismooth-pursuit relateding active or passive sinusoidischargemodulationwe testeduring-pursuit activessive head and whore-body rotation without a target in compassivearknesinusoidal
To ensure that the selected time windows did not contain artefacts related to mouth movements we tested, separately for each frequency band, that there was no amplitude difference in the EMG signal between the selected time windows and the baseline (−300 to 0 ms) (paired t-test, P < 0.05).
To minimize gaze-velocity related discharge modulation during active or passive head movement, we also tested these task conditions without a target in complete darkness.
To ensure our decoding accuracies could not result from spurious factors (e.g., task-correlated head or arm movements), we also tested the performance of our classifiers in ROIs outside of our action-related network where no statistically significant classification should be possible.
We tested whether movement behaviour of red-backed salamanders, Plethodon cinereus, is repeatable and whether it varies within and between forest stands of different ages in a fragmented landscape in New York, U.S.A. Water loss is a major constraint on P. cinereus mobility, and forest fragmentation creates spatial variation in desiccation risk.
If true, then vocal non-learning birds should have similar brain areas associated with movement control, an idea that we tested next.
To determine the influence on our results of the movement of fish among areas, we tested the sensitivity of our model results to the level of mobility, by changing the retention probability q: decreasing q results in an increase of movement between reserves and harvested areas.
To seek evidence that these small movements reflect intrinsic cytoskeleton dynamics, we tested the effect of actin-perturbing drug jasplakinolide on the neuron cells.
We tested whether movement-associated gene expression was specific to ZENK or a general IEG property, by examining expression of another IEG, c-fos, which is generally thought to be less sensitive to small changes in neural activity, and thus can reveal areas that were highly active during a specific behavior.
