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Conversely, the 127 rankable random ECoG events identified presented the opposite association, with 86.6% having no behavioural change, and only 13.4% having a behavioural change (P < 0.001, Fisher's Exact test), which also consisted of a movement arrest in all cases.
Of the 34 rankable EEEs lasting 0.8 2 s that were identified (Fig. 5C), 88.2% were associated with a behavioural change (a freeze-like movement arrest in all cases) while 11.8% were not.
In the latter case the movement arrest starts at the anterior region, such that cells from the posterior region, that are still moving forward, join the anterior portion of the organism, forming the early culminant (DDANAT:0000009).
We further observed perilesional quadruple spikes, longer multi-spike EEEs associated with movement arrest (Fig. 2D and E) that were electrographically similar to grade 1 seizures, and transitioned to obvious grade 2 seizures, that spread contralaterally, and were also associated with behavioural arrest (Fig. 2F).
They consisted of a behavioural sequence including (i) movement arrest, sometimes associated with limb jerks; (ii) hyperkinetic running, often with repeated, large clonics of all limbs and frequently incontinence and loss of postural equilibrium; and (iii) dystonic or hypertonic posture of the trunk, limbs and tail, often asymmetrically.
We found that all EEEs lasting ≥2 s were associated with a detectable change in behaviour which typically consisted of a movement arrest with or without facial automatisms, or a stereotyped loss of posture in which the animal crouched down and was motionless at the bottom of the cage, with or without motor manifestations such as facial automatisms or contralateral limb dystonia.
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