Most importantly, try the bindings with a gloved hand and move the bindings around a lot to see if you can manipulate them easily.
It is possible that moving the binding site for MxiE, the transcription factor that activates transcription of the ipaHs, closer to the ipaH4.5 transcription start site causes a greater number of ipaH4.5 messages to be successfully completed.
This causes the main chain of the loop to move toward the binding site and to fold with a different conformation compared to the apo structure which places the backbone NH groups of Ileb472 and Thrb471 at a good distance and orientation to serve as hydrogen bond donors to oxygen atoms of the pyrophosphate group.
Because only one face of the FMN is accessible within the protein environment, moving the substrate binding site to the opposite side of the cofactor appeared impractical.
The mutant pJM11 containing mutations that moved the αCTD-binding site one helical turn upstream had the largest defect, making only 15% of wild-type activity, implying that the location of the αCTD-binding site in Pm is important, possibly because the αCTD requires direct interaction with Mor.
The question is whether he'll move the parties to something like binding arbitration, stop speaking about psychological breakthrough, and start implementing American policy more Dr. Kissinger, less Dr. Phil.
The question is whether he'll move the parties to something like binding arbitration, stop speaking about psychological breakthrough, and start implementing American policy — more Dr. Kissinger, less Dr. Phil.
Protein movement consequent to agonist binding may move the two high-Φ domains (loop A and the αM2-cap) closer than they are in the unliganded-closed Torpedo AChR structural model.
In this system, the quencher completely blocks the fluorescence signal from the fluorophore and target binding can move the quencher away from the fluorophore and have "signal on" detection [ 265].
In addition, BbpSer547 Gln561 in the N3 domain moved toward the binding groove to make contact directly with the peptide, while BbpAsp338 Gly355 and BbpThr365 Tyr387 in N2 domain shifted their configurations to stabilize the reorganized C-terminus mainly through strong hydrogen bonds.
