Two lines of mice which label GFAP+ astrocytes and stem cells in the SVZ indicated complete lack of motility.
We therefore performed immunostaining using an antibody against the skeletal muscle marker 12/101 in newborn mice, which labelled individual muscle fascicles of the mLVP inferior to the ET and next to the nasopharynx.
The expression of PSCA-CAR was verified using Alexa-647 FragmentFragment of Goat Anti-Mouse IgG (H + L) (Invitrogen), which labels the heavy and light chain of mouse IgG and analyzed by flow cytometry analysis.
Therefore, different from the POMC-GFP mice, which only labels the new-born neurons up to two weeks postmitotic, our GAD67-GFP transgene labels the whole developmental stage of newborn neurons.
Sanford Barsky (Ohio State University, Columbus, OH, USA) presented elegant data from a transgenic mouse model in which labeled male bone marrow cells were transplanted into irradiated female recipients.
The difference in the number of proliferating OPCs in cuprizone- or cuprizone plus rapamycin-treated mice was determined by double labeling with rabbit Ki67 antibody, which labels all proliferating cells and rat anti-PDGFRα.
In postnatal Ret mutant mice, VGLUT1+ puncta, which label pre-synaptic terminals of mechanoreceptors and proprioceptors (Hughes et al., 2004; Paixão et al., 2013), are greatly reduced in layers III V, indicating deficits in the third order central projections of RA mechanoreceptors (Luo et al., 2009).
By labeling P15 SVZs with Olig2, which labels parenchymal oligodendrocytes precursors and SVZ precursor cells, we found a significant accumulation of Olig2 in mice lacking Dicer (control: 23.7±2.8% Gfap Cre Dicer f/f : 36.7±6%, P<0.05).
To conditionally delete Cxcl12 in nestin+ cells in the first postnatal week, we intercrossed Cxcl12 fl mice (Tzeng et al., 2010) with Nes-CreER T2 mice, which mostly label Nes-GFP+ cells during this period.
