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Amino acid sequences and the corresponding nucleotide alignments for orthologous genes in human and mouse were retrieved from Ensembl.
(2) E D = log 2 R i R t × D × f To investigate the expression at gene level, the coding sequences for the mouse were retrieved from the Consensus Coding Sequence Database (CCDS) [ 49].
The microarray data on gene expression in the heart and liver of the MRL/MpJ mouse were retrieved from GSE19322, GSE4710, GSE25322, and GSE5241 datasets deposited in the GEO database.
To identify new zebrafish miRNAs, existing miRNA sequences from mouse were retrieved from the miRNA registry [ 26] and compared with the zebrafish and fugu genome using BLAST and Ensemble's Zebrafish or Fugu Genome databases [ 32, 33].
Whole-genome sequence alignments for chicken, turkey, and zebra finch and for human, macaque, and mouse were retrieved from the Ensembl database release 73 via the Ensembl perl Application Program Interfaces.
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Following immunization, fewer than 3000 vaccine-associated cells per mice were retrieved from the draining LN, including 1500 2000 Adj+ (IC31®+) DCs and 500 Ag+Adj+ DCs.
The data for CO-related experiments on mouse models were retrieved from the Mouse Genome Informatics (MGI) database (http://www.informatics.jax.org/).org/
Each mouse was tested for 120 min. Sequences of previously reported human and mouse CD33rSiglecs were retrieved from HGNC (http://www.genenames.org/) and MGI (http://www.informatics.jax.org/), respectively.
The corresponding regulatory regions of human (ENSG00000111602) and target mouse (ENSMUSG00000039994) were retrieved using a genome browser such as EnsEMBL (www.ensembl.org), and the retrieved orthologous pairs of genomic sequences were re-aligned using the ORCA aligner [83].
Mouse orthologs were retrieved from Ensembl BioMart.
Complete amino acid sequences of currently known human and mouse chemokines were retrieved from Genbank.
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