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For all stainings, two sections per mouse were quantified.
(H ) Quantification of Ki-67-positive cells (n=2 and 2 mice; ten 20x fields for each mouse were quantified).
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Total bioluminescence emission from whole mouse or defined areas within the images of each mouse was quantified (in photon units) using the Living Image software package (Xenogen Corporation/Caliper Life Sciences).
Total photon emission was recorded for each animal, and the light emitted from each individual mouse was quantified using the Region of Interest (ROI) tool.
Distance travelled (cm) of each mouse was quantified (Garcia-Corzo et al, 2013).
The total photon flux of each mouse was quantified using Living Image® software.
(A ) The percentage of pSTAT5+ and total STAT5a+ cells in 15 early lesions per mouse was quantified.
Total photon emission from vaginal areas within the images (region of interest (ROI)) of each mouse was quantified with Living ImageR software package [ 19].
Total photon emission from vaginal areas within the images (Region Of Interest, ROI) of each mouse was quantified with Living ImageR software package.
Three months after injecting MCF-7/DOX cells through the tail veins of balb/c nude mice, the mice were killed, and the total number of visible lung tumor nodules per mouse was quantified under a stereomicroscope.
The cerebellar frataxin deficiency of the mouse frataxin knockin-knockout (KIKO) mouse was quantified by qRT PCR; the animals contained 31% of frataxin compared with wild type (WT) mice as control (P > 0.0032, n = 4).
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