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After BAL, whole lungs from each mouse were perfused with 20 mL chilled saline to remove red blood cells.
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The left atrium was snipped with scissors and the mouse was perfused with heparinized saline from a peristaltic pump through a 27 gauge needle.
Mouse was perfused with 10 ml PBS from the portal vein.
The left lung of each mouse was perfused with 10% neutral buffered formalin and submitted for histological processing.
For immunohistochemsitry mice were perfused by transcardial perfusion using chilled 4% paraformaldehyde.
Following pentobarbital euthanasia, mice were perfused of blood as above.
Anesthetized mice were perfused with 4% formaldehyde (PFA).
These mice were perfused with PBS before harvesting tissues for analysis.
Briefly, mice were perfused with heparinized cold saline followed by 10% formalin fix under anesthesia.
After 2 h, mice were perfused with 4% paraformaldehyde in PBS (pH 7.4) and brain tissue collected.
The mice were perfused after imaging, and iron stains and immunohistochemical studies were performed.
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