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Hepatic H22 tumor cells (5 to 6 × 106 cells per mouse) were inoculated subcutaneously to the Institute for Cancer Research ICRR) mice (6 to 8 weeks, 22 to 26 g) at the right axilla.
A total of 103 bacteria per mouse were inoculated.
After extensive wash with PBS, the cells (in 500 µl PBS per mouse) were inoculated i.p into C57BL/6 mice.
To grow parasites in sufficient quantities to prepare DNA for subsequent analysis, blood was, thereupon, collected, and 106 pRBC from each mouse were inoculated into four non-immune mice.
Ba/F3-JAK2V617F cells (10 per mouse) were inoculated intravenously into 7-week-old mice.
A total of 4 × 10 (0.2 ml) Bel7402 cells per mouse were inoculated subcutaneously into the right dorsal flanks of nude mice.
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After one week, each mouse was inoculated subcutaneously in the right flank with HT-29 human colon cancer cells.
On day zero, every mouse was inoculated with 2 × 106 live H22 cells subcutaneously in the right hind leg.
In brief, each experimental mouse was inoculated intra-peritoneally (i.p).
The concentration of parasites was adjusted and each mouse was inoculated intraperitoneally (i.p).
Then 10 12 weeks old female BALB/c mouse was inoculated with 3 × 106 hybridoma cells.
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