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For primary tumour growth assays and spontaneous metastasis via s.c. injection, cells (2 × 106 per mouse) were injected subcutaneously into the right upper flank region of NOD/SCID mice.
Both eyes of each mouse were injected with the same virus.
After washing and dead cell removal, 5×106 cells in 200 µl HBSS per mouse were injected i.v. into tail veins in seven C57/B6 mice per group.
We employed a transplant model, in which one million leukocytes from the enlarged spleen of a leukemic Eμ-Tcl1 mouse were injected into a cohort of C.B-17 SCID mice via tail vein, essentially as described by Wu et al. [44].
Two mg of Arthrogen-CIA per mouse were injected intravenously into the tail vein of mice.
A549 cells (2 × 10 per mouse) were injected subcutaneous in 100 μl serum-free medium.
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For intrasplenic injection, cells (3 × 10 each mouse) were injected into the spleen of nude mice.
Briefly, CMS4-met tumor cells (2.5×105 cells/mouse) were injected into mice i.v. in 100 µl HBSS.
Colon 26 cells (1.5×10 cells/mouse) were injected to BALB/c mice iv.
CMS4-met tumor cells (2×105/mouse) were injected i.v. into naive mice.
GFP-hAMSCs (0.5 × 10 cells/mouse) were injected into the striatum of athymic mouse 30 days after GBM276 brain tumor-initiating cells (BTICs; 0.3 × 10 cells/mouse) were injected into the ipsilateral deep striatum (schematic, Figure 7d).
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CEO of Professional Science Editing for Scientists @ prosciediting.com