Suggestions(5)
Exact(5)
Briefly, for NK cytotoxic activity, lymphocytes isolated from spleen of each mouse were incubated with YAC-1 or B16 cells at different effector/target (E T) ratios at 37°C for 4 hrs, and targeted cell lysis was calculated according to the manufacturer's instructions.
Both FDB muscles from each mouse were incubated in collagenase solution at 37°C for 2 h, and the mixture was manually shaken every 30 min to improve digestion of connective tissue.
A total of 100 μg of liver mitochondria isolated from a C57BL/6J mouse were incubated with 10 μM [H]PGZ (PGZ H(G) hydrochloride; Hartmann Analytic, Grupo Taper SA, Alcobendas, Spain) at 27°C for 30 minutes.
Milk samples collected from a transgenic mouse and a nontransgenic mouse were incubated with various ratios of tabun (GA), sarin (GB), soman (GD) and VX in the nerve agent binding and inhibition assay (Fig. 3).
Both FDB muscles from each mouse were incubated in collagenase solution at 37°C for 2 h, and the mixture was manually shaken every 30 min to improve digestion of the connective tissue.
Similar(55)
After blocking with 5% skim milk, primary antibody (glial fibrillary acidic protein (GFAP) Pharmingen, San Diego, USA, mouse) was incubated overnight at 4°C.
Fifteen microliters of serum or homogenised spleen tissue from each mouse was incubated for 3 hours at room temperature with 5 μL of nitrate reductase (5 U/mL; Boehringer Mannheim, Laval, Quebec, Canada) and 15 μL of NADPH (1.25 mg/mL; Boehringer).
ASCs isolated from green fluorescence protein (GFP) transgenic mice were incubated in an adipogenic medium and then seeded onto type I collagen sponge, non-woven polyglycolic acid or hyaluronic acid gel.
No signal was elicited when islets from mceph/mceph mice were incubated with an antibody against Kv1.2.
Macrophages isolated from heedless, oblivious, insouciant and Tlr1−/− mice were incubated with different concentrations of LTA for 4 hours.
Splenocytes from C57BL/6 mice were incubated with Salmonella infected DCs from the Balb/C mice for 72 hr as described above for cell proliferation assay.
More suggestions(15)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com