Statistical comparisons between AMD3100-treated mice and PBS-injected mock mice were done using the Student t test.
Handling and testing of the mice were done during the light phase of the light-dark cycle.
Statistical analysis between the different groups of vaccinated and unvaccinated mice as compared to the PBS control mice were done using Fisher's exact test.
These studies on the KO and D36 mice were done in parallel and therefore cannot be explained by different experimental conditions.
Injections of AAV-Cre or AAV-GFP into the rPCRt of these mice were done using a compressed air delivery system [37], [41].
All the experiments involving mice were done according to a protocol reviewed and approved by the Institutional Animal Care and Use Committee of Institute of Biosciences and Technology, in compliance with the USA Public Health Service Policy on Humane Care and Use of Laboratory Animals.
Analysis of CD41 positive platelets in kidney from healthy mice were done by manual counting of all positively stained platelets in sections from whole kidney from n = 5 6 individuals/group.
The leukocyte transfers in mice were done in MHC-matched and relatively young recipients [ 2].
Previous behavioural studies on Tc1 mice were done on B6129S8 background [34,19].
