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In the animals carrying E34 and U87 tumours, multiple ROIs (n=9 per mouse) were defined within the tumour.
The six goal locations in each maze were specified in allocentric coordinates, such that the baited arms for each mouse were defined by their relation to extramaze cues.
Conserved noncoding sequences between human and mouse were defined as regions with minimum 70% identity over 100 bp of sequence [ 28].
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Instruction about basic principles of genetics is minimal, with a 'knock-out' mouse being defined in terms of a transgenic mouse, for readers who know what the latter is.
The alternation score for each mouse was defined as the ratio of the actual number of alternations to the possible number (defined as the total number of arm entries minus two) multiplied by 100 as shown by the following equation: % Alternation = [(Number of alternations)/(Total arm entries − 2)] × 100.
Social recognition by a subject mouse is defined by decreased investigation of a previously encountered mouse.
The last stage completed by the mouse was defined as the peak velocity (vPeak).
Tumor burden per mouse was defined as the sum of the volume of all macroscopic tumors.
The flux of rolling leukocytes for each mouse was defined as the average of individual numbers of rolling leukocytes seen in all major veins.
Cells that express higher levels of CD24 in mouse are defined as luminal epithelial cells, and Lin-CD29hiCD24+ cells have been defined as mammary stem cells in mouse [ 48, 49].
The percentage of chemicals positive for an end point in both rat and mouse, over the total positive for the same end point in only the rat or mouse, was defined as "species concordance".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com