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Coronal cerebellum sections (20 µm) of wild type and CB2 knock-out mouse were cut in a cryostat microtome.
Five samples per mouse were cut into 4-μm-thick slices.
A total of 250 serial sections in each mouse were cut on gelatin coated slides and frozen at −80°C until use.
For immunohistochemical analysis, the entire brains (or the right brain half in case of the APP751SL/PS1M146L mouse) were cut into entire series of 30-μm-thick frontal sections on a cryostat (Leica CM 3050 S; Leica, Nussloch, Germany).
Here, paraffin-embedded tissue microarrays (human or mouse) or sagittal brain hemispheres (mouse) were cut into 5 μm sections, placed on glass slides and stained as previously described (72).
The colon tissues of mouse were cut into 0.5 1.0 mm tablets, placed into the sandwich structure, and covered with DMEM medium containing 20% bovine serum and HMQ18 22 (1.0, 4.0, 16.0 μmol/l) or vehicle alone.
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For the blood glucose measurement, the tail vein of each mouse was cut and the glucose content was measured by using a Glucose PILOT kit (Aventir Biotech, LLC, Carlsbad, CA, USA) under light ether anesthesia.
The glandular stomach of each mouse was cut for microarray analysis.
For fluorescence labelling, RF muscle from mouse was cut into 8 μm cryostat sections, dried for 30 min and treated as previously described by Mänttäri et al [ 4].
Rando's experiments involved an unsettling but remarkable procedure in which mice were cut along the flanks and sewn together, wound-on-wound.
Livers from individual mice were cut into 2 × 4 × 4 mm3 sections, fixed in 4% paraformldehyde and embedded in paraffin.
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CEO of Professional Science Editing for Scientists @ prosciediting.com