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Pairs of submandibular glands from the same mouse were cultured together, one implanted with an Fgf10 bead, the other with a control bead.
Unless otherwise specified, embryos from each mouse were cultured separately in microdrops of potassium simplex optimized medium with amino acids (KSOM/AA; Millipore, Billerica, MA) covered with mineral oil and embryo morphological appearance was documented daily.
(E ) CD4+ splenocytes from an untreated mouse were cultured with serum drawn from PBS, FK506 or FKDC treated mice after the last dose of treatment (day 14), and the CD4+ T cells then stimulated for 4 hr with CD3/28 beads.
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Furthermore, ovaries derived from Atg7 F/F ;Tnap-cre mice were cultured using the in vitro neonatal ovary culture system.
For immunocytochemistry and TUNEL assay, primary keratinocytes isolated from control and knockout mice were cultured on ColI/FN coated 8-well ibiTreat tissue culture slides (Ibidi).
Primary keratinocytes isolated from control and knockout mice were cultured to examine the effect of selenoprotein ablation on keratinocyte properties in culture.
Neural cells from cerebral cortex of embryonic day-14 mice were cultured on the film coated with poly-l-lysine.
(A) Single myofibers isolated from RNF13-/ and RNF13+/+ mice were cultured for 24 h or 72 h and stained for Pax7 (red), MyoD (green), and DAPI (blue).
Pleural lavages of saline and S. pneumoniae infected mice were cultured on blood agar plates containing 5%% sheep blood (Remel Blood Agar, Fisher Scientific).
After photodynamic therapy the mice were cultured again for colony-forming units per milliliter and then killed, their tissue harvested for histopathology.
Pleural lavage from euthanized saline and S. pneumoniae infected mice were cultured on blood agar dishes to determine if live S. pneumoniae persisted throughout the 7 days time course.
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