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In this study, two different low doses (LDs) of phosphorodiamidate morpholino oligomer (PMO) designed to skip the mutated exon 23 in the mdx dystrophic mouse were administered for up to 12 months.
In additional biodistribution studies, two ligand doses of the 177Lu-DOTA-PEG5k-Lys-BN 177Lu-DOTA-PEG5k-Lys-BN 177Lu-DOTA-PEG5k-Lys-BN 177Lu-DOTA-PEG5k-Lys-BN 177Lu-DOTA-PEG5k-Lys-BNspecific, 0.3 nmol; or low specific, 3.0 nmol peptide injected per mouse) were atministered.
For VSV strain Indiana, 10 PFU per mouse were administered intranasally.
The adenoviral vectors AdNK4 and AdLacZ (control) (10 PFU per mouse) were administered intravenously 1 day before 1833/TGL cells, and then every 5 days.
The animals were allowed to recover for 4 days before the experiments commenced, and icv injections of 10 nmol morphine per mouse were administered daily for 7 consecutive days.
Bispecific single chain FVS were radioiodinated as described above, and I-bs-scFvs (∼20 μg per mouse) were administered to cohorts of mice (n⩾5 per cohort) through tail-vein injection, mice were euthanized at indicated times post-injection and dissected, and major organs were weighed and counted in a γ well counter (Cobra Quantum, Packard Instruments) with a window of 15 75 keV.
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177Lu-DOTANOC (5 MBq, 2.5 nmol per mouse) was administered intravenously (i.v).v
Each mouse was administered diazepam (3 mg/kg, suspended in 0.5% carboxymethylcellulose solution) or vehicle i.p.
Each mouse was administered 200 cfu i.p. and the dose was enumerated by plating on LB agar media.
Each mouse was administered imipramine hydrochloride (20 mg/kg, dissolved in saline) or vehicle i.p. 30 min before the trial.
Each mouse was administered azithromycin once daily over the course of infection.
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