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Slices were then imaged at 200×magnification using a Nikon Optishot II microscope and Zeiss Axiocam digital camera; 12 images per mouse were acquired.
Axial scans of a mouse were acquired with bSSFP with 4 PC, 2 NEX, FA 50° and BW ±62.5 kHz (14 minutes) as determined above and compared to T1wSE (20 min) and T2wSE (17 min) scans with parameters as indicated in the methods section (Figure 3).
Five colour images per mouse were acquired under identical conditions (exposure time, white balance) in bright fields.
TreadScan data on each mouse were acquired for about 20 seconds of fast walking at 18 cm/second at a 17° uphill gradient.
Measurements of the XPM-2 Phantom Mouse were acquired using the instrument at Dartmouth college, from five light bands centred around 560nm, 585nm, 602nm, 630nm, and 650nm, with widths of 10nm, resulting in 1120 measurements.
Tomographic bioluminescence measurements of a Caliper XPM-2 Phantom Mouse were acquired and reconstructions from simulation and this experimental data show that Compressive Sensing-based reconstruction is superior to standard reconstruction techniques, particularly in the presence of noise.
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Before the simultaneous imaging, MRI of the left and right popliteal lymph node of an anaesthetized BALB/c mouse was acquired as the control.
An in vivo PAT-OCT image of the skin on the flank of a 5-week-old anaesthetized female hairless mouse was acquired.
In addition, 9 normal ICR mice were acquired from an AAALAC-qualified animal center (BioLASCO, Co., Ltd., Taiwan) for FUS system confirmation and all animals were then housed at Chang Gung University.
Dynamic coincidence planar images of six tumors and two inflammations in nude mice were acquired over 4 hours immediately after injection of 25.9 MBq of [18F]FDG into the right thigh of each animal.
Immediately after microPET imaging, optical images of the mice were acquired (excitation: 675 nm, emission 720 nm) for 1 s.
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