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For BrdU incorporation, mice were first injected intraperitoneally with BrdU (1 mg per 6 g of mouse weight), followed by administration of BrdU in the drinking water (0.8 mg/ml) for 5 days.
We induced K/BxN arthritis in BALB/c mice by transferring 1 μL of serum/g of mouse weight, followed by 0.5 μL of serum/g of mouse weight 48 hours later.
injection of 1 μL of K/BxN serum per 1 g of mouse weight, followed 48 hours later by i.p. injection of 0.5 μL of K/BxN serum per 1 g of mouse weight.
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Mice at 10 weeks were administrated with Bromodeoxyuridine (BrdU) (50 mg/kg mouse body weight), followed by immunohistochemical analysis with anti-BrdU (Dako).
6 Urine and fecal samples for metabolic analyses were collected between 06:00 and 08 00 hours, followed by mouse weight and blood measurements, to minimize metabolic variation caused by diurnal fluctuation, 1 day before the infection and at days 1, 3, and 4 postinfection.
"It's mostly mouse weight".
The mean mouse weight was approximately 21.8 ± 2.2 g.
Mouse weight was monitored weekly.
Body weights of LAB mice, followed from 3 to 16 weeks of age, were reduced by ∼20% as compared with control mice, despite undetectable levels of IGF1 in serum (Yakar et al., 2009b).
The DANA and Tamiflu treatments did not discernably affect mouse behavior or significantly affect mouse weights (Supplementary Fig. S10).
Mouse weights were 20 24 g.
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