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Mice were sacrificed by cervical dislocation and after worm recovery, liver and spleen were removed from each mouse, weighed and their relative weights (g of organ/100 g of body weight) were calculated.
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Organs were excised from sacrificed mice, weighed and homogenized in PBS.
Brains and spinal cords from Rat IgG2a and αB7-H1 treated mice at days 7 and 14 post-infection were collected from anesthetized mice, weighed, and homogenized on ice using a Polytron System PT1200C tissue homogenizer (Kinematica AG, Switzerland).
At the end of the treatment period, tumors were excised from mice, weighed and then fixed for 3 h at 4°C with 4% paraformaldehyde, embedded in paraffin wax and sectioned (6 µm).
Two cm of the distal colon was harvested from colitic mice, weighed and cultured overnight in RPMI 1640 supplemented with 10% heat-inactivated FBS, penicillin (100 U/ml), streptomycin (100 µg/ml), L-glutamine and Amphotericin B (1 µg/ml).
To determine bacterial numbers in the stools, faecal pellets were collected from individual mice, weighed, and homogenised in 1 mL of SPS.
Twenty-four hours before the test, each mouse was weighed and then deprived of all food in their home cage.
The uterus of each mouse was weighed and then fixed in 4% paraformaldehyde.
The pellets collected from each mouse were weighed and the bedding was changed to eliminate reinfection.
For 3 days before testing, mice were weighed and handled in order to habituate the mice to contact with the experimenter.
Twice weekly for the duration of the experiment, mice were weighed and tumor volumes were evaluated (eight observations of each type per mouse).
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