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In mouse we obtained 1860 ESTs and 105 mRNAs, from 50 tissues.
Similar to that of mouse, we obtained binary matrices of mRNA-miRNA interactions for orthologous 3′ UTR in other species.
For mouse, we obtained orthologs in human, chimp, macaque, rat, cow, dog, and horse from Biomart through the Ensembl Homology track (http://www.ensembl.org/).org/
From each mouse we obtained duplicate samples of adipose (inguinal fat pad), heart, kidney, and liver tissues by splitting whole organs or tissues prior to homogenization and RNA extraction.
In addition to the human and mouse, we obtained the sequence of the PEDF gene and surrounding region for chick, frog and several fish species from the genomic databases.
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From 128 Hdac6 transgenic mice we obtained thus far, only one female exhibited abnormal growth of the neck by the age of 10 months.
Additionally, there is a possibility that chromosomal rearrangement could have occurred during passage in the FLPe mice we obtained, resulting in loss of gene copy number of the functional FLPe gene.
Using a similar strategy for generation of Hand1 TG mice, we obtained Twist1-WT and -DD transgenic TG mice and found that while -WT TG mice had comparable heart to control mice, Twist1-DD TG mice displayed hypertrophy and some of them had atrial septal defect (ASD) and ventricular septal defect (VSD) (Figure 2B D).
In order to investigate the reconstitution of the human immune system in these mice, we obtained PBMC from the mice at 10, 12 and 17 weeks after the transplantation and then analyzed them by using flow cytometry for detecting human T and B cells.
For Sema3dLacz / knockin mice, we obtained the targeting vector from KOMP.
After crossing between heterozygous mutant mice, we obtained Got1l1 KO mice identified by Southern blotting (Fig. 2b).
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