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With a focus on potential target molecules linking TMEM135 to a genetic network involved in fat biology and survival in the mouse, we also sought to determine if there exists a link between TMEM135 and insulin signaling within this genetic network.

In the current study with the Cyp46a1−/− mouse we also observe both 22R-hydroxycholesterol and 20R,22R-dihydroxycholesterol 20R,22R-dihydroxycholesterol

Given the regulation of prostate differentiation by PPAR γ2 in mouse, we also wanted to confirm the expression of PPAR γ2 and some of its downstream-regulated genes in human prostate tissue.

As this assay has wide variability depending on how the plug develops and is adsorbed in the mouse, we also used the more reliable and quantitative Directed In Vivo Angiogenesis Assay (DIVAA).

Based on RegNetwork, we characterized the statistical and topological properties of genome-wide regulatory networks for human and mouse, we also extracted and interpreted simple yet important network motifs that involve the interplays between TF-miRNA and their targets.

To be able to compare the degree of MSCI and PSCR between dog and mouse, we also generated similar boxplots for mouse spermatocytes and spermatids, using the recently published RNA sequencing dataset, using purified spermatocyte and round spermatid cell fractions of similar purity to our samples (Additional file 1: Tables S2 and S3) [ 46].

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In addition to the smaller right ventricles found in Cdc42 knockout mice, we also noted that the inactivation of Cdc42 resulted in ventricle wall evagination.

To reveal any potential toxic effect of BN-PEG on the liver and heart of the treated mice, we also carried out the test of blood biochemistry.

Along with HDC−/− mice, we also used histamine H1-/H2-receptor gene-double knockout (H1R−/−/H2R−/−) mice generated by crossbreeding of H1-receptor null mice and H2-receptor null mice.

Moreover, in order to find out whether the reduction of GM-CSF could lead to the expression changes of other genes which lead to the enhanced metastasis in RNF13-KO mice, we also detected expression patterns of several genes related to metastasis (Fig. S6C).

For adult mice, we also tested the effect of previous participation to the challenge as pups.

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