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The left kidney from each mouse was weighted and homogenized in 4 volumes of sterile saline using a tissue homogenizer to correct for differences in weight (Polytron PT1300D homogenizer, Kinematica AG).
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Values from all slices of a mouse were weighted according to area and averaged.
Values from all slices of a mouse were weighted according to area and averaged, and the averaged values normalized by the corresponding baseline values.
Before injection of the melanoma cells, mice were weighted and grouped (adjusted to a mean body weight of 20 g (range: 18.5 21.0 g) into three treatment groups and one control group (20 mice per group).
On day 16 or 17, mice were weighted and sacrificed by decapitation.
F2 mice were weighted before the infection, and then every 7 days.
Mortality was recorded and all mice were weighted and graded blindly by one observer (I-M J) for arthritis severity and frequency at regular intervals.
Just as in the wild-type retinas, the tuning curves from the Cx57-deficient mice were weighted towards low spatial frequencies in the scotopic light condition and toward high spatial frequencies in the photopic light condition.
Treatments were administrated at about 9 o'clock daily by gavage, and mice were weighted daily.
In all experiments, mice were weighted daily during all treatment time.
Hearts and pancreases from mice were weighted, homogenized in 0.5 ml MEM, and centrifuged for 10 minutes at 1000 rpm.
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CEO of Professional Science Editing for Scientists @ prosciediting.com