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For imaging, the mouse was then placed in a custom-designed holder [5] and scanned in an Inveon preclinical PET tomograph (Siemens Preclinical Solutions, Knoxville, TN).
Specifically, each P-DIE mouse was then sacrificed together with one CLP mouse predicted to survive (henceforth referred to as P-SUR) that was defined by BT ≥ 35°C (Figure 1B).
After the injection of microcapsules for 1 hour, the tumor of each mouse was then irradiated by the MW at the power density of 1.8 W/cm2 for 5 min.
The mouse was then placed back in its cage.
The mouse was then awoken with an injection of 2.5 mg/kg Antisedan.
Each mouse was then immediately returned to its home cage from the treadmill.
The mouse was then removed from the platform and returned to its home cage.
Each mouse was then exposed to 5 consecutive presentations of the CS-only spaced by 90-s intervals.
The R26-Stop-HA3-ICA512-CCF mouse was then crossed with the RIP-CreER mouse [9] (Fig. 1A).
The mouse was then brought back to the home cage for 1-h post-training sleep/rest.
The anaesthetised mouse was then transferred to the imaging chamber and anaesthetic was continuously administered by nose cones throughout the imaging procedure.
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