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The mouse was subsequently ventilated with a Harvard pump ventilator (tidal volume = 0.4 ml, frequency = 120 breaths/min, positive end-expiratory pressure 2.5 3.0 cm H2O) and the tail vein was cannulated with a 27 gauge needle for injection of the methacholine challenge.

One WT mouse was subsequently removed from analysis due to an indeterminate genotyping result post-experiment.

The RecNciI mouse was subsequently used to study the role of GlcCerase in epidermal function (Doering et al., 1999).

Once the mouse vocalized, the experiment was terminated and the mouse was subsequently removed from the chamber.

Chemotherapy with 8 mg/kg doxorubicin was i.v. administered twice at days 0 and 3. Oncolytic HSV1-hGM-CSF treatment at a dose of 1 × 10 plaque forming units (PFU) per mouse was subsequently given by direct intratumoral injection on days 5, 7, 9, 11 and 13.

The virulence of the new H1N1 strain in SCID mice was subsequently analyzed.

UCP1 protein expression in the WAT of Tg mice was subsequently verified by both western blotting (Figure 4B) and histological immunostaining (Figure 4C).

Since PGC-1α regulates mitochondrial biogenesis and energy production through induction of UCP1 expression and through functional interaction with PPARγ2 and the retinoic acid receptor (RXRα), the thermometabolic capacity of PRLR KO mice was subsequently evaluated.

Liver chromatin from individual mice was subsequently used to perform ChIP-seq with an antibody recognizing CREB, regardless of phosphorylation status.

The heterozygous mutant mice were subsequently intercrossed to generate homozygous mutant mice (Fig. 1C).

LFA-1 FRET CD11a-mYFP/CD18-mCFP CD11a-mYFP/CD18-mCFP CD11a-mYFP/CD18-mCFPing the CD11a-mice and CD18-mYFP knock-in mice.