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The total volume collected from each individual mouse was less than 10%% of the total blood volume, namely each mouse was sampled about three times and each time a blood sample of approximately 0.25 mL was obtained.
Each mouse was sampled three times, reflecting three distinct stages of tumor progression: primary tumor (day 1 post-transplantation), hormone sensitive (HS) tumor (day 7 post-transplantation), and hormone refractory (HR) tumor (day 114 post- transplantation).
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For the analyses of all the mutant mice, 2 or 3 PC per each mouse were sampled.
In approach I, a group of mice was sampled at 6 hours and sacrificed at 24 hours to test the MA-MP6H6 efficacy.
Heart rate and activity for individual mice was sampled continuously in 2-s bins at 30-s intervals and recorded against a date/time stamp using the dataquest A.R.T.™ software (Data Sciences International).
The colon tumors induced on the backs of mice were sampled to form paraffin-embedded sections.
Three CLP-ODam mice were sampled at 24, 48 and 72 h post-CLP and were followed for 7 days after CLP (and euthanized on day 8 post-CLP) to ensure the CLP-ODam setup was not lethal in the acute phase of sepsis.
To achieve a large sample size (total N = 1,013), mice were sampled from generations 45 48.
All mice were sampled at 17 weeks of age in the main study.
Deer mice were sampled from 16 sites near the West Tintic Mountains in the Great Basin Desert of central Utah (Juab and Utah Counties) on public lands.
At each interval, at least 6 mice were sampled.
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