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The mouse was required to grasp the middle part of the wire with their forepaws, and the length of time for the mouse to fall off the wire was recorded.
For the first two routes 100 µg of CPMV-AF488 was used and detection was performed after 4 hours, while for the oral route the dose of 1 mg of labeled particles (1×1014 virus particles) per mouse was required to detect CPMV in APCs at 20 hrs post-dosing.
In the next phase of shaping the mouse was required to find the reward using only the EB odorant adsorbed to the filter paper on the dish wall with no odor added to the sugar pellet.
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Controls: A keyboard and mouse are required.
Therefore, cell-autonomous CXCR1/2 signalling in human, and CXCR2 signalling in mouse, are required for the maintenance of chondrocyte phenotypic stability.
The main restriction of our method is that PET/CT images (as opposed to PET only images) of the moving mouse are required since the high-resolution anatomical CT image serves as the basis for actual mouse to atlas registration.
Enhancers at −110 kb (77 kb in mouse) and in intron 5 (intron 4 in mouse) are required for appropriate haematopoietic expression (Martowicz et al, 2005; Grass et al, 2006; Khandekar et al, 2007; Brandt et al, 2008).
Although oral administration of disease associated autoantigens such as GAD65 can lead to the induction of tolerance in the murine model, dosage on the order of milligrams per week per mouse are required [ 65].
In a subcutaneous xenograft model of glioblastoma, expression of PLA2G4A by the host mice was required for tumor growth [ 56].
Immunocompetent mice are required to examine the full effect of the NS1 mutation on viral replication through IFN production and signaling.
All previous NASA missions in which mice were co-housed, involved female mice; however, in our spaceflight studies examining bone healing, male mice are required for optimal experimentation.
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