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Total photon emission was recorded for each animal, and the light emitted from each individual mouse was quantified using the Region of Interest (ROI) tool.
Total bioluminescence emission from whole mouse or defined areas within the images of each mouse was quantified (in photon units) using the Living Image software package (Xenogen Corporation/Caliper Life Sciences).
Distance travelled (cm) of each mouse was quantified (Garcia-Corzo et al, 2013).
The total photon flux of each mouse was quantified using Living Image® software.
(A ) The percentage of pSTAT5+ and total STAT5a+ cells in 15 early lesions per mouse was quantified.
Total photon emission from vaginal areas within the images (region of interest (ROI)) of each mouse was quantified with Living ImageR software package [ 19].
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For all stainings, two sections per mouse were quantified.
(H ) Quantification of Ki-67-positive cells (n=2 and 2 mice; ten 20x fields for each mouse were quantified).
Latent viral load in the spleens of infected mice was quantified by real-time PCR using the ABI 7300 Real Time PCR System (Applied Biosystems, Foster City, CA, USA).
Latent viral load in the spleens of infected mice was quantified by real-time PCR using the ABI 7300 Real Time PCR System (Applied Biosystems, Foster City, CA).
Interaction between the male and female mice was quantified by manual annotation of video recordings in which the behaviors were categorized as sniff body, approach, chase, walk away, mount and attempt mount, thrust and/or ejaculation, or non-interacting.
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