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After 3 min, each mouse was positioned directly facing the slit lamp, holding the animal gently by the scruff of the neck.
The mouse was positioned on a 37°C heated platform in order to restrain the knee joint and the distal tendon was attached to the lever arm of a servomotor (Model 305B-LR, Aurora Scientific, ON, Canada).
The left hindlimb was shaved, aseptically prepared, and each mouse was positioned on a heated platform with its left foot placed in a metal foot plate attached to the servomotor and knee clamped to maintain positioning throughout the experiment.
Each mouse was positioned in ventral recumbency and the lumbar area was prepared aseptically for surgery.
The trial started when a mouse was positioned inside the starting shelter box.
The anesthetized mouse was positioned in a ventral position so that a dorsal lateral vein could be located.
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Next, the mouse is positioned along the edge of the table, with its forelimbs suspended over the edge and allowed to move freely.
The mouse is positioned in a stereotactic head holder/gas mask (Fig. 2a).
Adult male Sprague-Dawley rats (weight 380 450 g), wild-type (WT) mice, and αβγ−/− mice were anesthetized with urethane (1.3 g/kg IP), and an octapolar electrophysiology catheter (1.6F for rats and 1.1F for mice) was positioned in the right atrium and the right ventricle via the jugular vein or in the left ventricle via the right common carotid artery.
The mice were positioned in a prone position into the center of the field of view.
The mice were positioned in the Siemens Inveon microPET/CT as described above, whereupon a 10-min PET acquisition was obtained, followed by a CT scan.
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CEO of Professional Science Editing for Scientists @ prosciediting.com