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Each mouse was maintained under general anesthesia (air and 2% isoflurane).
In either case (whether it reached the platform by itself or not), the mouse was maintained on the platform for 30 additional seconds.
Capsaicin (50 µl) was administered as 0.3% solution (dissolved in 10% ethanol, 10% Tween 80 and 80% saline) instilled into the colon via a Hamilton syringe catheter (rounded-tip needle 3.8 cm long) while the mouse was maintained under Isoflurane anesthesia.
The mouse was maintained in a cylinder and the tail immersed into a heated water bath set at 52 °C.
MBT-2, which was established from N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced N-[4- 5-nitro-2-furyl -2-thiazolyl]-formamide-induced
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The core body temperature of the mice was maintained using a warming pad set to 37 °C.
The temperature of the mice was maintained by an air-warming system at approximately 37 °C.
During scanning, body temperature of the mice was maintained using a heated bed.
All mice were maintained in specific pathogen-free housing.
ICR mice were maintained in the animal facility of National Yang-Ming UNational Yang-Ming
All mice were maintained on the C57BL/6 background.
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