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On day 50, each intruder mouse was introduced to a resident mouse in the resident's home cage temporarily divided by an acrylic board with several holes.
In another test, a younger mouse was introduced three times to a Shank3 mutant and to a wild-type mouse.
Thus wired, a mouse was introduced to another, lightly anesthetized mouse.
When a new mouse was introduced into their cage, they greeted the new guy much more eagerly than unmodified mice did.
Next an unfamiliar, same sex virgin mouse was introduced into the wire cage.
On day 1 (pretest), the mouse was introduced in the central alley and left free to explore the entire apparatus.
Similar(32)
The test consists of analysis of behaviors manifested by the test mouse in his own cage in three consecutive 10-min sessions: undisturbed, nonsocial engaging (a novel object is introduced), and social engaging (a non-familiar mouse is introduced).
A group of mice was introduced 0.2 ml SWF (containing SEB 50 μg and 10 mg horseradish peroxidase, HRP. SWF was concentrated with the method of ammonium sulfate precipitation for higher content of SEB) via intragastric gavage under a light anesthetization.
Then, somehow or other, mice were introduced.
On the next day, mice were introduced to two identical objects (two shot glasses, 1.5" wide × 3" tall, or two white glass vases, 3" wide × 4" tall ) for 10 min in the arena, returned to their home-cage for 60 min, and then exposed to one object from the first session (vase or shot glass) and one novel object (shot glass or vase) for 10 min.
Pathogen-Free mice were introduced into Germ-Free mouse cage (ratio 1∶1) for 4 days and maintained in sterile cages.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com