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The average amount of LG recovered (8.01 μg) was about 24% of that instilled, assuming that each mouse was instilled with 33.25 μg LG (26.6% of 125 μg).
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To confirm that VEGF is capable of inducing inflammation and neuronal plasticity, another group of mice was instilled with recombinant VEGF165 or VEGF121 into the urinary bladder.
While the untreated group was not subjected to any treatment, another group of mice was instilled with 10 μg of naked pDNA in 100 μL of buffered DMEM solution.
Negative control mice were instilled with sham (saline) injections, followed by instillation of free MSN following the same timing as tumor mice.
Two control mice were instilled with distilled water and treated identically.
In the second experiment 6 mice were instilled intratracheally with 2 nm gold and 6 mice were instilled with distilled water to examine whether the gold instillations were toxic and to quantify the gold with greater sensitivity.
Two mice were instilled intratracheally with 50 μl 2, 40 or 40 nm gold nanoparticles and were killed after 1 h.
Because the mice were instilled with an accumulated dose of 3.03 μg gold, 1.9 ± 0.6‰ of the instilled 2 nm gold particles had accumulated in the liver 1 day after the 5th injection.
Female C57BL/6 mice were instilled weekly for two weeks with VEGF (6.41 nM in 100 μl).
For this purpose, all mice were instilled with 2 mL of PD solution (dextrose 4.25%).
Eight 8 weeks old female mice were instilled intratracheally once as described in detail previously [Jackson et al., 2011a].
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