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These were randomized so that each mouse was implanted with two different matrigel preparations (e.g. one from the negative control preparation and one containing normoxic mASCs).
Each athymic mouse was implanted subcutaneous with a 0.72-mg of 17-β-estradiol pellets, 2 weeks before inoculation of MCF-7 cells [17], [18].
Each BALB/c mouse was implanted intradermally on the ventral surface with the cells.
Since each mouse was implanted with two xenografts, each group had 20 tumors.
To supplement the estrogens for MCF-7 proliferation each nude mouse was implanted with a 1.5 mg of 17β-estradiol pellet (Innovative Research of America, Sarasota, FL, USA).
Each mouse was implanted with bilateral optic fibers, and stimulation was delivered either to one side ('unilateral #1'), the contralateral side ('unilateral #2') or to both sides ('bilateral'bilateral
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Small tumor fragments (∼1 mm3, estimated using 1 mm×1 mm grid) from a donor mouse were implanted into the right cerebral cortex of anesthetized recipient mice.
One group of mice was implanted with 12 human liver buds each, and a second group was a control.
A second group of 32 mice was implanted simultaneously with a pellet of 17β-estradiol and an implant of LNG (20 µg/day) (LNG group).
One group of 32 mice was implanted subcutaneously in the neck with a pellet of 17β-estradiol (E2; 1.72 mg/60 day release; Innovative Research of America) (= E2 group).
All mice were implanted with custom-made fiber optic cannulas 100 µm above the injection site.
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